FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_85.3510000005bd24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_85.3510000005bd24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21124
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA840.3976519598560878No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC310.14675250899450862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGTC455.677375E-721.04513221
CTCTGTC250.003231755221.0451320
AATTTTT557.8143785E-920.99526219
GGATTTT354.3415526E-520.99526218
TAGTTTT354.3415526E-520.99526218
ACCGACT1100.020.9952628
ACCGACA1100.020.9952628
ACCGAAC950.020.9952628
ACCGAAA1100.020.9952628
TACCGAC4400.020.9952627
CGAGCGT354.3415526E-520.99526210
CCGAGGC701.2732926E-1120.9952629
CCGAGCG701.2732926E-1120.9952629
CCGAGAC354.3415526E-520.9952629
CACTAGT354.3415526E-520.9952621
ACTAGTA354.3415526E-520.9952622
CCGATTG354.3415526E-520.9952629
CCGATTA354.3415526E-520.9952629
CCGATGT354.3415526E-520.9952629
CCGACCG354.3415526E-520.9952629
Produced by FastQC (version 0.11.2)