FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_79.3510000005bc75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_79.3510000005bc75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25730
Sequences flagged as poor quality0
Sequence length27
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAAAAAAAAAAAAAAAAA790.3070345899727944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGTA303.7912774E-421.00000210
ATCTTTT303.7912774E-421.00000218
AGCTTTT303.7912774E-421.00000218
GCAAGAT303.7912774E-421.0000029
GCAAGAA303.7912774E-421.0000029
CAATTCA303.7912774E-421.00000210
GCAATTG303.7912774E-421.0000029
GCAACGG609.331416E-1021.0000029
GCAATGG609.331416E-1021.0000029
GCAACAT303.7912774E-421.0000029
GCAATCC303.7912774E-421.0000029
CTGGTTT303.7912774E-421.00000217
GCAGTTT303.7912774E-421.00000217
TCGTTTT303.7912774E-421.00000218
CAAAGCG303.7912774E-421.00000210
CAAGGTG303.7912774E-421.00000210
CAAGGTC303.7912774E-421.00000210
GGGGTTT609.331416E-1021.00000217
CAAGGCT303.7912774E-421.00000210
CAAGGCG303.7912774E-421.00000210
Produced by FastQC (version 0.11.2)