FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_78.3510000005bc5c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_78.3510000005bc5c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18362
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC920.5010347456704063No Hit
AGTGTGACGAACCTGTCTCTTCTACAC330.17971898486003704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGAC354.2165244E-521.0491812
GCGTGAC659.822543E-1121.0491812
AGAGTGA354.2165244E-521.0491811
AGTGACG354.2165244E-521.0491813
AGCGTGA659.822543E-1121.0491811
CGTGACG659.822543E-1121.0491813
GTGTGAC16100.021.0491812
TGTGACG16300.021.049183
GGTGACG751.8189894E-1221.049183
GGGTGAC751.8189894E-1221.049182
AACGTGG455.7329817E-720.99182710
GAACGGT455.7329817E-720.9918279
CATTTTT900.020.99182719
GAGTTTT455.7329817E-720.99182718
GAACTCG455.7329817E-720.9918279
GAACCAG455.7329817E-720.9918279
CGAACCG1750.020.9918278
CGAACAG1750.020.9918278
GCGTTTT455.7329817E-720.99182718
GACGAAC18250.020.9918276
Produced by FastQC (version 0.11.2)