FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_77.3510000005bc32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_77.3510000005bc32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21455
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTCCAAGCACTGTCTCTTATACAC2771.291074341645304No Hit
AAGGTCCAAGCCTGTCTCTTATACACA2311.0766721044045677No Hit
AAGGTCCAAGCACTGTCTCTTCTACAC940.43812631088324405No Hit
AAGGTCCAAGCAGTGGTATCAACGCAG580.2703332556513633No Hit
AAGGTCCAAGCAAAAAAAAAAAAAAAA340.15847121883010953No Hit
AAGGTCCAAGCACCTGTCTCTTATACA250.11652295502213937No Hit
AAGGTCCAAGCCTGTCTCTTCTACCCC230.10720111862036821No Hit
AAGGTCCAAGCCTGTCTCTTATACCCA220.10254020041948264No Hit
AAGGTCCAAGCTGTCTCTTATACACAT220.10254020041948264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCCA609.076757E-1021.0000022
GCCTTTT303.7629085E-421.00000218
TTTCTGT303.7629085E-421.00000221
AGGACCA455.8066144E-721.0000022
GGACCAA455.8066144E-721.0000023
GGTCTGT455.8066144E-721.00000219
TGATTTT303.7629085E-421.00000218
GCAGGGG455.8066144E-721.00000210
GCAGGGC303.7629085E-421.00000210
GTATTTT455.8066144E-721.00000218
GGATTTT455.8066144E-721.00000218
ATGGTCC609.076757E-1021.0000021
TAGTTTT303.7629085E-421.00000218
AGCAGTC455.8066144E-721.0000029
AGCAGTA303.7629085E-421.0000029
AGCAGCG609.076757E-1021.0000029
AGCAGAG303.7629085E-421.0000029
GCAGTGG303.7629085E-421.00000210
GCACGGG303.7629085E-421.00000210
GAATTTT303.7629085E-421.00000218
Produced by FastQC (version 0.11.2)