FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_76.3510000005bc18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_76.3510000005bc18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18229
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGACGAGATCTGTCTCTTATACAC1610.8832080750452576No Hit
AGAGGACGAGCTGTCTCTTATACACAT950.5211476219211147No Hit
AGAGGACGAGATCTGTCTCTTCTACAC650.35657468868286796No Hit
AGAGGACGAGCTGTCTCTTATCCACAT370.20297328432717102No Hit
AGAGGACGAGATGCTGTCTCTTATACA290.15908716879697185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATTT250.003255316821.00000217
GTGTTTT250.003255316821.00000218
GCCTTTT250.003255316821.00000218
AGCTTTT250.003255316821.00000218
ACGTTTT250.003255316821.00000218
GATACCG250.003255316821.00000210
AGGACGA16000.021.0000023
CGCTTTT250.003255316821.00000218
AGGGTTT250.003255316821.00000217
CATTTTT651.0186341E-1021.00000219
GATCGCT250.003255316821.00000210
TCTATTT250.003255316821.00000217
ATCGGGG250.003255316821.00000211
AGATGTT250.003255316821.0000029
GATTTTG250.003255316821.00000210
AGATGGT506.5903805E-821.0000029
AGATGGC651.0186341E-1021.0000029
AGATGGA506.5903805E-821.0000029
AGATGCC250.003255316821.0000029
AGGTTTT250.003255316821.00000218
Produced by FastQC (version 0.11.2)