FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_74.3510000005bbc6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_74.3510000005bbc6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26126
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC3781.4468345709255148No Hit
ACTGTCTCTGTCTCTTATACACATCTC2701.0334532649467962No Hit
ACTGTCTGTGTCCTGTCTCTTCTACAC1270.48610579499349305No Hit
ACTGTCTGTCTCTTATACACATCTCCG540.20669065298935924No Hit
ACTGTCTGTGTCTGTCTCTTATACACA450.17224221082446606No Hit
ACTGTCTCTGTCTCTTATACCCATCTC390.14927658271453725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGTCT651.05501385E-1021.038391
ACAGTCT303.746703E-421.0383871
ACCGTCT900.021.0383871
ACGGTCT1050.021.0383871
TTGTTTT651.09139364E-1020.99808518
TGGTTTT800.020.99808518
TGCATTT303.7958173E-420.99808517
ACTGTGT303.7958173E-420.9980855
GTGTCTG1550.020.9980858
GTGTCTC1550.020.9980858
GTGTCTA1600.020.9980858
GTGTTTT701.2732926E-1120.99808518
GGGTTTT1100.020.99808518
CAGTCTG303.7958173E-420.9980852
GTGTCGT1600.020.9980858
GTGTCGG2800.020.9980858
GTGTCGC2400.020.9980858
GTGTCGA1650.020.9980858
GTGTCCT1750.020.9980858
GTGTCCG1400.020.9980858
Produced by FastQC (version 0.11.2)