FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_69.3510000005bb33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_69.3510000005bb33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23887
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC350.14652321346339015No Hit
ACCTTGAACTGGAGCTGTCTCTTATAC280.1172185707707121No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT250.003284975221.00000218
GCCTTTT506.813934E-821.00000218
CAGTTTT506.813934E-821.00000218
ATCTTGA405.053913E-621.0000021
ACGTTTT250.003284975221.00000218
CTGGGTA250.003284975221.0000029
TTTCTGT405.053913E-621.00000221
CTGGGGT506.813934E-821.0000029
CTGGGAG800.021.0000029
GAAGTTT250.003284975221.00000217
TTATCTG250.003284975221.00000218
CTCTTTT250.003284975221.00000218
GCGGTTT250.003284975221.00000217
GGATCTG250.003284975221.00000218
CTCTTAT405.053913E-621.00000219
CATTTTT557.925337E-921.00000219
CCGTTTT250.003284975221.00000218
TAGTCTG250.003284975221.00000218
CTGGTTG405.053913E-621.0000029
CTGGCGC651.07320375E-1021.0000029
Produced by FastQC (version 0.11.2)