FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_65.3510000005baba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_65.3510000005baba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTGCGTATGCTGTCTCTTATACAC130.506427736657577No Hit
AACGTGCGTATGCCTGTCTCTTATACA100.38955979742890534No Hit
AACGTGCGTATGGGCTGTCTCTTATAC90.3506038176860148No Hit
AACGTGCGTATGGCTGTCTCTTATACA70.2726918582002337No Hit
AACGTGCGTATCTGTCTCTTATACACA60.23373587845734323No Hit
AACGTGCGTATGCTGTCTCTTCTACAC50.19477989871445267No Hit
AACGTGCGTATGCGAGGACGTTTTTTT40.15582391897156214No Hit
AACGTGCGTATGGTCTGTCTCTTATAC40.15582391897156214No Hit
AACGTGCGTATGGGGGCATGTTTTTTT30.11686793922867161No Hit
AACGTGCGTATGAGGTGGGGTTTTTTT30.11686793922867161No Hit
AACGTGCGTATGGCTGGTGGTTTTTTT30.11686793922867161No Hit
AACGTGCGTATGGCCTGTCTCTTATAC30.11686793922867161No Hit
AACGTGCGTATGGGAGCTGTCTCTTAT30.11686793922867161No Hit
AACGTGCGTATGGTGTGTAGTTTTTTT30.11686793922867161No Hit
AACGTGCGTATGCCGGGCGATTTTTTT30.11686793922867161No Hit
AACGTGCGTATGGGGCGAAGTTTTTTT30.11686793922867161No Hit
AACGTGCGTATGTCTGTCTCTTATACA30.11686793922867161No Hit
AACGTGCGTATGGGGGGGCATTTTTTT30.11686793922867161No Hit
AACGTGCGTATGAAGCGGCGTTTTTTT30.11686793922867161No Hit
AACGTGCGTATGACAACGGGTTTTTTT30.11686793922867161No Hit
AACGTGCGTATGGGGGTGCATTTTTTT30.11686793922867161No Hit
AACGTGCGTATGTGGAAGAATTTTTTT30.11686793922867161No Hit
AACGTGCGTATGGGCCATTATTTTTTT30.11686793922867161No Hit
AACGTGCGTATGAGCTGTCTCTTATAC30.11686793922867161No Hit
AACGTGCGTCTGTCTCTTATACACATC30.11686793922867161No Hit
AACGTGCGTATGTCGCGGGGTTTTTTT30.11686793922867161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAT2500.021.05
TTTTTTT1950.021.021
TGTTTTT352.7053582E-521.019
GGTTTTT302.6474395E-421.019
CGTATGT302.6474395E-421.07
CGTATGG1050.021.07
CGTATGA701.8189894E-1221.07
GCGTATG2550.021.06
GTGCGTA2500.021.04
AGTTTTT352.7053582E-521.019
CTTTTTT302.6474395E-421.020
GTATGGG502.602792E-820.9999988
GTATGGC250.002551460920.9999988
AACGTGC2450.020.9999981
GTATGAT250.002551460920.9999988
GTTTTTT1100.020.99999820
CGTATGC502.602792E-820.9999987
CGTGCGT2450.020.9999983
ATTTTTT552.4738256E-920.99999820
ACGTGCG2450.020.9999982
Produced by FastQC (version 0.11.2)