FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_63.3510000005ba77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_63.3510000005ba77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25535
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA2070.8106520462110828No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT860.3367926375562953No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC490.19189347953788916No Hit
TGGCATAGCTGAAGGGCTGTCTCTTAT380.14881535147836303No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT330.1292343841785784No Hit
TGGCATAGCTGAGGGGGCTGTCTCTTA260.10182102995887996No Hit
TGGCATAGCTGAGGGCTGTCTCTTATA260.10182102995887996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGGG303.7901528E-421.010
TTGTTTT354.380304E-521.018
TGGTTTT850.021.018
TTGCATA250.003291182221.01
GTGTTTT1000.021.018
GGGTTTT1700.021.018
GAGCGGG250.003291182221.011
GCCTTTT455.8972E-721.018
CAGTTTT405.0768667E-621.018
CTGAGTT455.8972E-721.09
CTGAGTG1000.021.09
CTGAGTC250.003291182221.09
GAGCGCG303.7901528E-421.011
GGCATTT303.7901528E-421.017
TGCATAG250.003291182221.02
CTGAGGT800.021.09
CTGAGGG2600.021.09
CTGAGGC1150.021.09
CTGAGGA701.2732926E-1121.09
CTGAGCT303.7901528E-421.09
Produced by FastQC (version 0.11.2)