FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_62.3510000005ba5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_62.3510000005ba5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19873
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCTGTTGCTGTCTCTTATACACA1740.8755598047602274No Hit
ATGCTCTGTTGCGGGGCTGTCTCTTAT440.2214059276405173No Hit
ATGCTCTGTTGCGGCTGTCTCTTATAC430.21637397473959646No Hit
ATGCTCTGTTGCGCCTGTCTCTTATAC320.16102249282946712No Hit
ATGCTCTGTTGCCTGTCTCTTATACAC280.14089468122578372No Hit
ATGCTCTGTTGCGGGGGGGGTTTTTTT220.11070296382025865No Hit
ATGCTCTGTTGCAGCTGTCTCTTATAC220.11070296382025865No Hit
ATGCTCTGTTGCTGCTGTCTCTTATAC210.10567101091933778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGA354.3172033E-521.00000211
TGTTGCC3350.021.0000027
GGGTTTT1300.021.00000218
TTGCAGC701.2732926E-1121.0000029
TTGCACG354.3172033E-521.0000029
GAGTTTT651.03682396E-1021.00000218
GAATTTT354.3172033E-521.00000218
GATTTTT1300.021.00000219
TTGCGTA354.3172033E-521.0000029
GGGGTTT701.2732926E-1121.00000217
TTGCGAT354.3172033E-521.0000029
TGCGGGT354.3172033E-521.00000210
GTTGCTC701.2732926E-1121.0000028
GGCGGTT354.3172033E-521.00000216
GTTGCCG1400.021.0000028
GTTGCAT354.3172033E-521.0000028
TTGCTGC354.3172033E-521.0000029
ACTGTTG303.749141E-421.05
TGGTTTT250.003265702221.018
TGTTGCG9350.021.07
Produced by FastQC (version 0.11.2)