FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_60.3510000005ba0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_60.3510000005ba0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24349
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCTTACGCCTGTCTCTTATACAC2060.8460306378085342No Hit
GAATCCTTACGCCTGTCTCTTCTACAC830.3408764220296521No Hit
GAATCCTTACGCTGTCTCTTATACACA340.13963612468684544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT751.8189894E-1221.00000218
GTGTTTT609.258656E-1021.00000218
ACGCCTG1050.021.0000029
GGCATTT303.7830113E-421.00000217
ACGCCGG609.258656E-1021.0000029
ACGCCCG455.8733895E-721.0000029
ACGCTCT303.7830113E-421.0000029
ACGCTCG303.7830113E-421.0000029
ACGCCAG455.8733895E-721.0000029
AAATTTT303.7830113E-421.00000218
ACGCAGG455.8733895E-721.0000029
ACGCAGA303.7830113E-421.0000029
TGATTTT303.7830113E-421.00000218
GGATTTT609.258656E-1021.00000218
GTATCCT751.8189894E-1221.0000021
CATTTTT751.8189894E-1221.00000219
TAGTTTT455.8733895E-721.00000218
GAGTTTT455.8733895E-721.00000218
TACGCGC2350.021.0000028
TATCCTT751.8189894E-1221.0000022
Produced by FastQC (version 0.11.2)