FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_59.3510000005b9ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_59.3510000005b9ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31390
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCCTCAGCTGTCTCTTATACACA680.2166294998407136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTTT900.021.00000218
GTGTTTT800.021.00000218
CAGTCTC455.9878766E-721.0000029
CAGTTTG405.1391926E-621.0000029
CAGTTTA405.1391926E-621.0000029
CAGTCGC405.1391926E-621.0000029
CAGTTCG405.1391926E-621.0000029
CAGTTAA405.1391926E-621.0000029
AATTTTT1600.021.00000219
CAGTATT405.1391926E-621.0000029
CAGTATA455.9878766E-721.0000029
CAGTAGT455.9878766E-721.0000029
CAGTAGC405.1391926E-621.0000029
CAGTAGA405.1391926E-621.0000029
TAGTTTT800.021.00000218
AGTGTGG405.1391926E-621.00000210
GTGGTTT405.1391926E-621.00000217
CGGTTTT405.1391926E-621.00000218
TACTTTT405.1391926E-621.00000218
CAGTGTC405.1391926E-621.0000029
Produced by FastQC (version 0.11.2)