FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_58.3510000005b9d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_58.3510000005b9d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21586
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC1990.9218938200685629No Hit
ACGTCTCTGCATCTGTCTCTTCTACAC850.39377374224034095No Hit
ACGTCTCTGCCTGTCTCTTATACACAT740.3428147873621792No Hit
ACGTCTCTGCCTGTCTCTTATCCACAT280.12971370332622997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTTT3600.021.04418820
GTTCTGT303.7102503E-421.04418620
TTCTGTC354.269225E-521.04418621
TGCATAG1650.020.9953618
ACTTTTT609.094947E-1020.99536119
GCATGTC303.769325E-420.9953619
GCATGCA303.769325E-420.9953619
GCGTTTT609.094947E-1020.99536118
GCATGAA609.094947E-1020.9953619
CATGGGT303.769325E-420.99536110
CGGTTTT609.094947E-1020.99536118
GCATCTT303.769325E-420.9953619
GCATTTA303.769325E-420.9953619
GCATCGA303.769325E-420.9953619
GACTTTT303.769325E-420.99536118
GCATTAC303.769325E-420.9953619
GCATATG609.094947E-1020.9953619
GCATACT303.769325E-420.9953619
CATGTGG303.769325E-420.99536110
GAGGTTT303.769325E-420.99536117
Produced by FastQC (version 0.11.2)