FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_55.3510000005b96b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_55.3510000005b96b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26788
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC1240.46289383305957893No Hit
CTTCAGCAATGCCTGTCTCTTCTACAC440.16425265044049575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGC1300.021.0000021
GTGTTTT651.09139364E-1021.00000218
AAATTTT354.3907305E-521.00000218
TGCGCGT354.3907305E-521.00000210
GGACTTT354.3907305E-521.00000217
TGCTGGG651.09139364E-1021.00000210
ATGCGTC354.3907305E-521.0000029
CCGTTTT354.3907305E-521.00000218
GAGTTTT701.2732926E-1121.00000218
ATGCTTG701.2732926E-1121.0000029
CCTTTTT701.2732926E-1121.00000219
ATGCTCG354.3907305E-521.0000029
ATGCCAC354.3907305E-521.0000029
GAATTTT354.3907305E-521.00000218
ATGCAGC354.3907305E-521.0000029
ATGCACC354.3907305E-521.0000029
AGATTTT354.3907305E-521.00000218
GTCAGCA1300.021.0000022
CTACAGC250.003295378321.01
TTGTTTT506.893606E-821.018
Produced by FastQC (version 0.11.2)