FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_53.3510000005b928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_53.3510000005b928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22836
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTACGTCTGTCTCTTATACACATC1330.5824137327027501No Hit
GAGCTACGTCTAGGGGCTGTCTCTTAT380.16640392362935716No Hit
GAGCTACGTCTAGGCTGTCTCTTATAC370.162024873007532No Hit
GAGCTACGTCTAGGGCTGTCTCTTATA350.1532667717638816No Hit
GAGCTACGTCTCTGTCTCTTATACACA300.13137151865475566No Hit
GAGCTACGTCTAAGCTGTCTCTTATAC250.10947626554562971No Hit
GAGCTACGTCTAAGGCTGTCTCTTATA240.10509721492380451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTT651.07320375E-1021.00000218
TCTAGTG751.8189894E-1221.0000029
TCTAGCG651.07320375E-1021.0000029
CATTTTT1300.021.00000219
TCTATTG651.07320375E-1021.0000029
GTCTAGG3000.021.0000028
CGTCTAT5050.021.0000027
CGTCTAG8250.021.0000027
TTGTTTT506.780465E-821.018
TGGTTTT250.003280563721.018
TAGCGGA250.003280563721.011
TCCTTTT354.3539632E-521.018
GTGTTTT609.1677066E-1021.018
ACGCTTT250.003280563721.017
GCCTTTT506.780465E-821.018
AGCTTTT250.003280563721.018
TCTAGTC354.3539632E-521.09
TCTAGTA354.3539632E-521.09
TCTAGGG1050.021.09
TCTAGGC701.2732926E-1121.09
Produced by FastQC (version 0.11.2)