FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_52.3510000005b90e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_52.3510000005b90e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18491
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGTCGATACCTGTCTCTTATACAC3101.6764912660213076No Hit
AGACGTCGATACCTGTCTCTTCTACAC1030.5570277432264346No Hit
AGACGTCGATACGGCTGTCTCTTATAC270.14601698123411389No Hit
AGACGTCGATACGCTGTCTCTTATACA250.13520090855010544No Hit
AGACGTCGATACACCTGTCTCTTATAC230.12438483586609701No Hit
AGACGTCGATACCCTGTCTCTTATACA210.11356876318208858No Hit
AGTCGTCGATACCTGTCTCTTCTACAC190.10275269049808014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1150.021.0434819
TTGTTTT354.2269792E-521.04347818
TGGTTTT303.6828147E-421.04347818
TCCTTTT250.003217839221.04347818
GTGTTTT455.600741E-721.04347818
AAATTTT250.003217839221.04347818
TATACAC354.2269792E-521.04347821
AATTTTT800.021.04347819
GGCGTTT303.6828147E-421.04347817
TTTCTGT250.003217839221.04347821
TTATTTT404.8619913E-621.04347818
CTCTTTT250.003217839221.04347818
CGCTTTT354.2269792E-521.04347818
TGTTTTT1250.021.04347819
TTCTGTC354.2269792E-521.04347821
GGATTTT659.822543E-1121.04347818
CATTTTT1350.021.04347819
GAGTTTT354.2269792E-521.04347818
TGCTTTT404.8619913E-621.04347818
CCATTTT354.2269792E-521.04347818
Produced by FastQC (version 0.11.2)