FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_5.3510000005b32e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_5.3510000005b32e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43434
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTACGTCTGTCTCTTATACACATC2630.605516415711194No Hit
GAGCTACGTCTCTGTCTCTTATACACA1040.23944375374130863No Hit
GAGCTACGTCTAGGGGCTGTCTCTTAT980.2256296910254639No Hit
GAGCTACGTCTAGGCTGTCTCTTATAC950.21872265966754156No Hit
GAGCTACGTCTAGGGCTGTCTCTTATA730.1680710963761109No Hit
GAGCTACGTCTAGCCTGTCTCTTATAC630.14504765851636967No Hit
GAGCTACGTCTAAGGCTGTCTCTTATA560.12893125201455083No Hit
GAGCTACGTCTAAGGGCTGTCTCTTAT550.12662890822857667No Hit
GAGCTACGTCTATGCTGTCTCTTATAC540.12432656444260257No Hit
GAGCTACGTCTACCCTGTCTCTTATAC530.12202422065662845No Hit
GAGCTACGTCTAGGCCTGTCTCTTATA490.11281484551273195No Hit
GAGCTACGTCTAGGGCCTGTCTCTTAT460.1059078141548096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTT456.0475395E-721.01845618
TGCATTT354.4426888E-521.01845617
GTGTTTT1050.021.01845618
CAGTTTT405.175696E-621.01845618
ATCTTTT354.4426888E-521.01845618
AGGATTT354.4426888E-521.01845617
GGCGTTT354.4426888E-521.01845617
GGATTTT1150.021.01845618
CGGATTT354.4426888E-521.01845617
GAGTTTT701.2732926E-1121.01845618
TAATTTT558.312782E-921.01845618
CACTTTT456.0475395E-721.01845618
GCGTTTT950.021.01845618
GGGATTT405.175696E-621.01845617
AGGTTTT900.021.01845618
GATTTCT354.4426888E-521.01845619
TGGGTTT456.0475395E-721.01845617
GTGGTTT405.175696E-621.01845617
GCCGTTT354.4426888E-521.01845617
CTGTTTT405.175696E-621.01845618
Produced by FastQC (version 0.11.2)