FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_49.3510000005b8bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_49.3510000005b8bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21257
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCTGCTCCTGTCTCTTATACACAT960.4516159382791551No Hit
GTGTCTGCTCCTGTCTCTTATCCACAT390.18346897492590675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTT557.64885E-921.0353818
TTGTTTT354.2793643E-521.03537818
TGGTTTT404.937274E-621.03537818
GTGTTTT404.937274E-621.03537818
GCCTTTT250.00324121421.03537818
CAGTTTT506.6045686E-821.03537818
GAAATTT250.00324121421.03537817
AAATTTT455.70677E-721.03537818
AATTTTT1200.021.03537819
GTGTATG1550.021.0353781
GTTCTGT250.00324121421.03537820
TGATTTT354.2793643E-521.03537818
CGCTTTT250.00324121421.03537818
TGTTTTT1550.021.03537819
AGGGTTT404.937274E-621.03537817
GGATTTT506.6045686E-821.03537818
TAGTTTT303.7183706E-421.03537818
GAGTCTG354.2793643E-521.0353781
GAGTTTT608.8584784E-1021.03537818
ACTTTTT608.8584784E-1021.03537819
Produced by FastQC (version 0.11.2)