FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_47.3510000005b879.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_47.3510000005b879.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80218
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGACTGGAGGCTGTCTCTTATAC1690.21067590815029044No Hit
TAGCAGACTGGAGCTGTCTCTTATACA880.1097010645989678No Hit
TAGCAGACTGGACCTGTCTCTTATACA870.10845446159216136No Hit
TAGCAGACTGGAGCCTGTCTCTTATAC870.10845446159216136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGTG558.6711225E-921.00000210
GAGGTGT303.8898044E-421.00000211
GATGGAG303.8898044E-421.00000211
TCCTTTT303.8898044E-421.00000218
GGAGAGA303.8898044E-421.00000210
GGATGGT558.6711225E-921.00000210
ATCTTTT601.02591E-921.00000218
TGACTGG303.8898044E-421.0000025
GAGTGAG303.8898044E-421.00000211
TAAGTTT303.8898044E-421.00000217
GGACTTA303.8898044E-421.00000210
TCGGTTT303.8898044E-421.00000217
GGACCCA303.8898044E-421.00000210
TGATTTT651.2187229E-1021.00000218
CGCTTTT651.2187229E-1021.00000218
TTTTCTG850.021.00000221
GTATTTT558.6711225E-921.00000218
GGATTTG303.8898044E-421.00000210
TAGCTGA303.8898044E-421.0000021
GAGCTTT303.8898044E-421.00000217
Produced by FastQC (version 0.11.2)