FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_46.3510000005b850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_46.3510000005b850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115423
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA3690.31969364857957255No Hit
TACTGCAAGGAGGGGGGGCTGTCTCTT1440.12475849700666244No Hit
TACTGCAAGGAGGCTGTCTCTTATACA1250.10829730642939449No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTCT303.8937968E-421.00823620
CAGTCTC303.8937968E-421.00823621
TTTTTTC405.3230688E-621.00823621
GTTTTTC303.8937968E-421.00823620
ATACACA354.5429602E-521.00823621
CTTTCTC303.8937968E-421.00823621
AGGGGTA405.343116E-620.99913411
CGTTTCT405.343116E-620.99913419
TTGTTTT751.8189894E-1220.99913418
TGGTTTT800.020.99913418
CTACAAG1400.020.9991343
TCCTTTT354.558179E-520.99913418
GAGGCAT558.776624E-920.99913410
GAGGTCA558.776624E-920.99913410
GGAGAGA2000.020.9991349
GAGGTAT601.0404619E-920.99913410
GAGCGTC558.776624E-920.99913410
AGGGGCG751.8189894E-1220.99913411
GGAGACA701.4551915E-1120.9991349
AGGGGAA303.9052212E-420.99913411
Produced by FastQC (version 0.11.2)