FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_45.3510000005b836.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_45.3510000005b836.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82851
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTGT651.200533E-1021.01147520
GTTTCTG507.3086994E-821.01147320
ATTTTCT250.003343118821.01147320
CTACAGT456.2428626E-720.99879510
CTACAGC456.2428626E-720.99879510
CTATATA303.8928157E-420.99879510
TAGTGTT303.8928157E-420.99879511
ACGCTAG29000.020.9987957
ACGCTAC18700.020.9987957
CTATAGT303.8928157E-420.99879510
CTATAGG303.8928157E-420.99879510
AGCTCGA3600.020.9987951
CTATACG303.8928157E-420.99879510
CTATACC303.8928157E-420.99879510
TAGGAAG456.2428626E-720.99879511
GCTAGGT2550.020.9987959
GGTCTTT303.8928157E-420.99879517
GAAGTTT456.2428626E-720.99879517
CTAAGTG456.2428626E-720.99879510
CTAAGTA303.8928157E-420.99879510
Produced by FastQC (version 0.11.2)