FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_43.3510000005b7f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_43.3510000005b7f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266381
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGGATGCTGTCTCTTATACACA18370.6896137487283251No Hit
CTAGCTGGATGTCTGTCTCTTATACAC4940.1854486618790379No Hit
CTAGCTGGATGTGGGGGCTGTCTCTTA3250.1220056986046302No Hit
CTAGCTGGATGTGGGCTGTCTCTTATA3040.11412225346402334No Hit
CTAGCTGGATCTGTCTCTTATACACAT2920.10961742766939084No Hit
CTAGCTGGATGTGGGGCTGTCTCTTAT2700.10135858037923125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCACA354.573609E-521.00713720
CTTTTCT250.003366429621.00713520
TTCACTC250.003366429621.00713521
CAGTCTC507.480048E-821.00713521
ATTTTTC456.326791E-721.00713520
ATTCACT250.003366429621.00713520
AACGTTT250.00337376920.9992517
GATGGAT4750.020.999254
TCCTTTT751.8189894E-1220.9992518
GAGCGTT507.50988E-820.9992516
TTTTGCG250.00337376920.9992512
TGGCATG250.00337376920.9992512
CAGTTTC250.00337376920.9992518
GTTTGGA250.00337376920.9992511
TCAAGTT250.00337376920.9992516
GTTTGCG250.00337376920.9992511
GTTTGAA250.00337376920.9992511
GTGCATG250.00337376920.9992511
TGGCAAG250.00337376920.9992512
TAGGACT250.00337376920.9992516
Produced by FastQC (version 0.11.2)