FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_41.3510000005b7a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_41.3510000005b7a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74559
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACCGATAGGCTGTCTCTTATACAC4190.5619710564787618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTT751.8189894E-1221.00000218
AGGGGAG405.299402E-621.00000210
CAGTTTT800.021.00000218
AGCTTTT558.647476E-921.00000218
TAGGATC800.021.0000029
TAGGAGG1700.021.0000029
TAGGAGA1300.021.0000029
TAGGACA1500.021.0000029
TAGGAAT1100.021.0000029
TGCGTTT405.299402E-621.00000217
AACGATA1500.021.0000024
AGGATGG558.647476E-921.00000210
AGGACAG558.647476E-921.00000210
AGGATAA405.299402E-621.00000210
TTATTTT1100.021.00000218
ATAGGGT5450.021.0000028
CACCGAT1100.021.0000023
ATAGGCA4200.021.0000028
AGGGCTA405.299402E-621.00000210
AGGGCGG651.2187229E-1021.00000210
Produced by FastQC (version 0.11.2)