FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_39.3510000005b76d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_39.3510000005b76d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48431
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCCTGTCTCTTATACACA1070.22093287357271169No Hit
ACTTCGTCAGCTGTCTCTTATACACAT710.14660031797815448No Hit
ACTTCGTCAGCTCTGTCTCTTATACAC490.10117486733703619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT405.158141E-621.03623221
ATTTCTG507.062408E-821.03623220
GTGTTTT800.021.01447918
ACGTTTT456.088612E-721.01447918
GAAGTTT405.2047617E-621.01447917
GCGGTTT558.390998E-921.01447917
GGATTTT900.021.01447918
CATTTTT1250.021.01447919
TAGTTTT800.021.01447918
GAGTTTT456.088612E-721.01447918
CGGGTTT405.2047617E-621.01447917
CCTTTTT1050.021.01447919
GCGTTTT651.1641532E-1021.01447918
CGGTTTT1050.021.01447918
AGATTTT651.1641532E-1021.01447918
AGTTCTG456.088612E-721.01447919
AAGTTTT651.1641532E-1021.01447918
GGTGTTT456.088612E-721.01447917
GCCTTTT609.877112E-1021.01447718
ATCTTTT303.841077E-421.01447718
Produced by FastQC (version 0.11.2)