FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_38.3510000005b744.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_38.3510000005b744.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72981
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA1660.2274564612707417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCC850.021.0240231
TGTTACC1200.021.0240231
TGTTGCC63600.021.0240211
TGTTCCC1000.021.0240211
TGGTGCC3300.021.0240211
GTTCTGT2250.021.00960420
TGTTCTG456.190912E-721.00960419
GGTTCTG900.021.00960419
CATTTTT2850.021.00960419
TTTGCCG850.021.0096042
TTGCCGA64150.021.0096043
CGTTTCT250.003341052421.00960219
TTACCGA1200.021.0096023
GTTCCCG1000.021.0096022
TTTTCTG250.003341052421.00960221
TTTTTCT250.003341052421.00960221
TGTTTCT250.003341052421.00960219
GTTTCTG1350.021.00960220
TTCCCGA1000.021.0096023
GCTGCCG1600.021.0096022
Produced by FastQC (version 0.11.2)