FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_37.3510000005b72a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_37.3510000005b72a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45935
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA1820.3962120387504082No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC750.16327419179275063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTGT651.1277734E-1021.05573820
TTTCTGT609.604264E-1021.05573721
TTCTGTC1200.021.05573721
ATTTCTG250.00328028121.05573720
CTTCTGT354.389272E-521.05573720
AGTTTTT2750.021.03275319
TGGTTTT1050.021.03275118
TCCTTTT303.814337E-421.03275118
TGCATTT250.003301177921.03275117
GGGTTTT1350.021.03275118
GCCTTTT405.155858E-621.03275118
GTCATTT250.003301177921.03275117
ATCTTTT354.4265194E-521.03275118
TTCGTTT303.814337E-421.03275117
TGCGTTT303.814337E-421.03275117
TTACTTT303.814337E-421.03275117
CGCCTTT250.003301177921.03275117
TGTCTTT303.814337E-421.03275117
CCTGTTT456.023529E-721.03275117
GGCGTTT250.003301177921.03275117
Produced by FastQC (version 0.11.2)