FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_36.3510000005b700.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_36.3510000005b700.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83900
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA3130.3730631704410012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT1350.021.01133221
TATACAC250.003343621.0113320
TTTTCTG558.632924E-921.0113321
TTTTTTC250.003343621.0113321
ATACACA250.003343621.0113321
CTTTCTC303.8777007E-421.0113321
CGACAGC507.358358E-820.998816
TTGTTTT1100.020.9988118
AACGCTG250.003355192320.9988117
TCCTTTT250.003355192320.9988118
ACAGTAA2000.020.998818
GCCTTTT558.691131E-920.9988118
CAGTCGG2750.020.998819
CAGTCCC1000.020.998819
CAGTCCA558.691131E-920.998819
CAGTTCT507.358358E-820.998819
CAGTTCA507.358358E-820.998819
CAGTTAG1000.020.998819
CAGTTAA250.003355192320.998819
TAGGAGG250.003355192320.9988112
Produced by FastQC (version 0.11.2)