FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_35.3510000005b6f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_35.3510000005b6f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242025
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA20620.8519781014358021No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCC303.9004552E-421.01654620
TCTACCC250.00335681821.01654620
TATCTTA250.00335681821.01654621
ATACCCA250.00335681821.01654621
ATTTTCT800.021.01654620
ATACACC303.9004552E-421.01654621
GTGTTTC354.5701723E-521.00785818
CCTATTT354.5701723E-521.00785817
CACATTT701.4551915E-1121.00785817
CAGTTCT354.5701723E-521.00785818
CCAGTTT701.4551915E-1121.00785817
TATGTTT701.4551915E-1121.00785817
ATAGTTT701.4551915E-1121.00785817
GATCTTT354.5701723E-521.00785817
AGGCTTT651.2551027E-1021.00785817
CGAGTTT701.4551915E-1121.00785817
CAGATTT651.2551027E-1021.00785817
ACCTTTT1400.021.00785818
GCCGTTT850.021.00785817
TTAATTT354.5701723E-521.00785817
Produced by FastQC (version 0.11.2)