FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_34.3510000005b6d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_34.3510000005b6d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69824
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC3300.4726168652612282No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA303.882819E-421.00000211
GATGGAG1100.021.00000210
TCCTTTT303.882819E-421.00000218
ATGGGAG303.882819E-421.00000211
CGATAGC1200.021.0000029
CGATAGA1100.021.0000029
CGATACT405.2913965E-621.0000029
CGATACG1200.021.0000029
CGATAAT601.0186341E-921.0000029
ACGTTTT800.021.00000218
GATACAA303.882819E-421.00000210
GATATAG303.882819E-421.00000210
CACGTTT303.882819E-421.00000217
TCGGTTT405.2913965E-621.00000217
TGATTTT1600.021.00000218
GATGTTG558.623829E-921.00000210
CATCTGT303.882819E-421.00000219
GCGGTTT405.2913965E-621.00000217
GACGGTT303.882819E-421.00000216
GATGCGA405.2913965E-621.00000210
Produced by FastQC (version 0.11.2)