FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_31.3510000005b66e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_31.3510000005b66e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63895
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAAAAAAAAAAAAAAAAA730.11424994130996165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTG250.003345412721.00000212
TGGTTTT1600.021.00000218
CAATGTC405.279704E-621.00000210
CAATGTA456.193204E-721.00000210
CAAGAGT507.284689E-821.00000210
CAAGAGA456.193204E-721.00000210
GGGTTTC354.515839E-521.00000218
CAATGGT456.193204E-721.00000210
CAATGGC354.515839E-521.00000210
CAAGAAG354.515839E-521.00000210
CAAGAAA250.003345412721.00000210
CAATGCA354.515839E-521.00000210
CAATGAG456.193204E-721.00000210
GTCATTT250.003345412721.00000217
ATCTTTT456.193204E-721.00000218
TCGATTT250.003345412721.00000217
TGCGTTT456.193204E-721.00000217
GCAAGGC1600.021.0000029
GCAAGGA2000.021.0000029
TGACTTT250.003345412721.00000217
Produced by FastQC (version 0.11.2)