FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_3.3510000005b2e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_3.3510000005b2e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250387
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC22530.8998070986113497No Hit
GAGGAACAGAGCTGTCTCTTATACACA4360.17413044606948444No Hit
GAGGAACAGAGTGGGGGCTGTCTCTTA2770.11062874670010824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCATTT303.9097058E-421.00959417
GGCATTT1150.021.00959417
TTCGTTT303.9097058E-421.00959417
TTACTTT303.9097058E-421.00959417
TGACTTT303.9097058E-421.00959417
GATATTT601.0513759E-921.00959417
CCGCTTT303.9097058E-421.00959417
CGCTTTT1200.021.00959418
TACATTT303.9097058E-421.00959417
ACTTTTT5500.021.00959419
CCACTTT303.9097058E-421.00959417
CCTTCTG303.9097058E-421.00959419
GGGATTC303.9097058E-421.00959417
GCCATTT601.0513759E-921.00959417
AGCATTT601.0513759E-921.00959417
ACGATTT303.9097058E-421.00959417
AATGTTT303.9097058E-421.00959417
ACAATTT303.9097058E-421.00959417
ACTATTT601.0513759E-921.00959417
CTTTTTC303.9097058E-421.00959420
Produced by FastQC (version 0.11.2)