FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_29.3510000005b62b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_29.3510000005b62b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84299
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTCCAAGCCTGTCTCTTATACACA8581.0178056679201413No Hit
AAGGTCCAAGCACTGTCTCTTATACAC7180.8517301510100951No Hit
AAGGTCCAAGCAGTGGTATCAACGCAG3160.374856166739819No Hit
AAGGTCCAAGCAGTGGTATCCTGTCTC1830.21708442567527492No Hit
AAGGTCCAAGCACCTGTCTCTTATACA890.10557657860710093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTCTG751.8189894E-1221.01128421
TTTTTCT751.8189894E-1221.01128421
AACGCAG303.8779445E-421.01128221
GTTTCTG950.021.01128220
GTTTTTC303.8779445E-421.01128220
GTTTTCT405.2882533E-621.01128220
ATTTCTG950.021.01128220
TGGTTTT1700.020.99881418
TCCTTTT601.029548E-920.99881418
TGCATTT303.8935462E-420.99881417
GGGTTTT2050.020.99881418
ACGCTTT250.00335530320.99881417
CATGAAC354.5399458E-520.99881411
TCGATTT250.00335530320.99881417
AAATTTT507.359267E-820.99881418
GCCCAAG601.029548E-920.9988144
TTACTTT303.8935462E-420.99881417
TGGTATC850.020.99881414
GATATTT303.8935462E-420.99881417
GCAAGCG507.359267E-820.99881410
Produced by FastQC (version 0.11.2)