FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_25.3510000005b5bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_25.3510000005b5bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81802
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC1740.2127087357277328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTT303.8784754E-421.00978717
TCCTTTT800.021.00978718
AATCTTT405.288399E-621.00978717
ACGCTTT303.8784754E-421.00978717
GGCATTT850.021.00978717
TAAATTT354.51959E-521.00978717
AGCTTTT1050.021.00978718
GAAATTT354.51959E-521.00978717
TGCGTTT601.020453E-921.00978717
CCAGTTT354.51959E-521.00978717
ACGTTTT850.021.00978718
AGGATTT354.51959E-521.00978717
TAAGTTT405.288399E-621.00978717
GGACTTT456.209448E-721.00978717
TTTCTGT800.021.00978721
GAAGTTT601.020453E-921.00978717
CCGCTTT303.8784754E-421.00978717
TGATTTT1400.021.00978718
AGGGTTT701.4551915E-1121.00978717
GAGCTTT354.51959E-521.00978717
Produced by FastQC (version 0.11.2)