FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_23.3510000005b57c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_23.3510000005b57c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63967
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA3520.550283740053465No Hit
ATCTGCTATCCTGTCTCTTATACACAT1090.17040036268701048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCTA751.8189894E-1221.0000022
TCCGGGG4550.021.0000029
TCCGTAA751.8189894E-1221.0000029
TCATTTT751.8189894E-1221.00000218
ATCCGGA4550.021.0000028
TCCGATT751.8189894E-1221.0000029
ATCCGCG4650.021.0000028
CCGCGAT250.003345470221.010
CCGCGAG405.2799205E-621.010
ACTGCTA1050.021.02
TGGTTTT1450.021.018
TCCTTTT250.003345470221.018
TTCATTT405.2799205E-621.017
TGCATTT405.2799205E-621.017
GGTTGTT405.2799205E-621.016
CCGGATG303.8778977E-421.010
CCGGATA303.8778977E-421.010
GGGTTTT3450.021.018
CAGTTTT1050.021.018
GTCATTT303.8778977E-421.017
Produced by FastQC (version 0.11.2)