FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_22.3510000005b553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_22.3510000005b553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81271
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGAACTACCGCTGTCTCTTATACA2770.340834984188702No Hit
GTCAGAACTACCCCTGTCTCTTATACA2680.3297609233305853No Hit
GTCAGAACTACCTCTGTCTCTTATACA1010.1242755718521982No Hit
GTCAGAACTACCGGGGGGCTGTCTCTT870.10704925496179449No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAA250.00334179221.0123161
GACAGAA2200.021.0123161
GTCAAAA507.3012416E-821.0123161
GTCCGAA3400.021.0123161
GCCAGAA1050.021.0123161
GTAAGAA558.616553E-921.0123161
GTTAGAA2000.021.0123161
GTCAGAA67050.021.0123161
GGCAGAA4100.021.0123141
ACCGGTT701.4551915E-1120.99938610
ACCGGTG1150.020.99938610
ACCGGTA303.89118E-420.99938610
GGATGTT303.89118E-420.99938616
TCCTTTT303.89118E-420.99938618
ACCGGCC601.027729E-920.99938610
GTGTTTT2700.020.99938618
ACCGGAT303.89118E-420.99938610
GTGTTTC303.89118E-420.99938618
ACCGGAC354.5364308E-520.99938610
ACCGGAA354.5364308E-520.99938610
Produced by FastQC (version 0.11.2)