FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_2.3510000005b2cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_2.3510000005b2cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78244
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAGGGCTGTCTCTTATA1160.1482541792341905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAGTG1300.021.0127961
GTTAGTG751.8189894E-1221.0127961
GGTCGTG3750.021.0127961
GGAGATT354.5336663E-520.99936116
TGGTTTT1400.020.99936118
CTACAAG456.231985E-720.99936110
TAGCGGG405.3063013E-620.99936111
TCCTTTT456.231985E-720.99936118
GGTGATT354.5336663E-520.99936116
TAGCGCG354.5336663E-520.99936111
TAGCGAG354.5336663E-520.99936111
GCCTTTT751.8189894E-1220.99936118
CAGTTTT651.2187229E-1020.99936118
GGCATTT456.231985E-720.99936117
CTATATG354.5336663E-520.99936110
GGGTTCT354.5336663E-520.99936118
CTGAGCT354.5336663E-520.9993615
CTATAGG558.6656655E-920.99936110
TAGGAGC354.5336663E-520.99936111
TCGATTT354.5336663E-520.99936117
Produced by FastQC (version 0.11.2)