FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_16.3510000005b497.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_16.3510000005b497.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44948
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC5271.172465960665658No Hit
GAACACCATAGCGGCTGTCTCTTATAC3770.8387469965293227No Hit
GAACACCATAGCCTGTCTCTTCTACAC2150.47833051526208065No Hit
GAACACCATAGCAGCTGTCTCTTATAC1940.4316098602829937No Hit
GAACACCATAGCGCCTGTCTCTTATAC1910.42493548100026696No Hit
GAACACCATAGCCCCTGTCTCTTATAC1700.37821482602118006No Hit
GAACACCATAGCGTCTGTCTCTTATAC1560.3470677227017887No Hit
GAACACCATAGCACCTGTCTCTTATAC1370.30479665391118627No Hit
GAACACCATAGCTGCTGTCTCTTATAC1340.2981222746284596No Hit
GAACACCATAGCCGCTGTCTCTTATAC1070.2380528610839192No Hit
GAACACCATAGCTGTCTCTTATACACA960.2135801370472546No Hit
GAACACCATAGCGACTGTCTCTTATAC930.20690575776452788No Hit
GAACACCATAGCATCTGTCTCTTATAC910.20245617157604343No Hit
GAACACCATAGCTCCTGTCTCTTATAC810.18020824063362106No Hit
GAACACCATAGCTTCTGTCTCTTATAC660.14683634421998754No Hit
GAACACCATAGCCCTGTCTCTTATACA650.1446115511257453No Hit
GAACACCATAGCTACTGTCTCTTATAC550.12236362018332296No Hit
GAACACCATAGCGCTGTCTCTTATACA540.12013882708908072No Hit
GAACACCATAGCAACTGTCTCTTATAC540.12013882708908072No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTCTG303.8020912E-421.04129620
TTTCTGT800.021.04129421
TTTTTCT354.4099063E-521.04129421
GTTTCTG405.1335956E-621.04129420
ACTTTTT1450.021.01783819
CCTTTTT1050.021.01783819
TATTTTT1500.021.01783819
GATTTTT2100.021.01783819
CGTTTTT1900.021.01783819
AATTTTT1600.021.01783619
AGTTTTT3200.021.01783619
TAGCGGT1150.020.9944349
TAGCAGC1150.020.9944349
CATAGCG17000.020.9944347
CATAGCC9200.020.9944347
CGGTTTT1150.020.99443418
AGCCTGG303.859959E-420.99443210
TGGTTTT751.8189894E-1220.99443218
TAGCGGG2850.020.9944329
TAGCGGA1200.020.9944329
Produced by FastQC (version 0.11.2)