FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_15.3510000005b47d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_15.3510000005b47d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63766
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA4330.6790452592290562No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT2010.315215004861525No Hit
TGGCATAGCTGAAGGGCTGTCTCTTAT1170.1834833610387981No Hit
TGGCATAGCTGAGGGCCTGTCTCTTAT1070.16780102248847348No Hit
TGGCATAGCTGAGGGGGCTGTCTCTTA1000.15682338550324623No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC960.1505504500831164No Hit
TGGCATAGCTGAGAGGCTGTCTCTTAT760.11918577298246716No Hit
TGGCATAGCTGACGGGCTGTCTCTTAT750.1176175391274347No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT690.1082081359972399No Hit
TGGCATAGCTGAGGGCTGTCTCTTATA650.10193520057711007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTGT1750.021.01491421
TTTTCTG405.2470223E-621.01491421
TTCTGTC2100.021.01491421
TTTTTCT250.003331586521.01491421
GTTTCTG1300.021.01491420
ATTCTGT354.4909866E-521.01491420
ATTTCTG250.003331586521.01491420
ATTTTCT250.003331586521.01491420
GTTCTGT1550.021.01491220
TGAGACC250.00334680520.99843210
TGATGGA250.00334680520.99843210
TGAGAAC250.00334680520.99843210
TGATGAC250.00334680520.99843210
GTGTTTT1500.020.99843218
CTGAGTG2050.020.9984329
CTGAGTA850.020.9984329
GAGCGCG250.00334680520.99843211
GTCATTT354.518297E-520.99843217
GGGTTCT354.518297E-520.99843218
CTGAGGG7550.020.9984329
Produced by FastQC (version 0.11.2)