FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_14.3510000005b454.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_14.3510000005b454.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59228
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCTGTTGCTGTCTCTTATACACA3990.6736678597960425No Hit
ATGCTCTGTTGCGGCTGTCTCTTATAC1500.2532585939082866No Hit
ATGCTCTGTTGCGGGGCTGTCTCTTAT1380.23299790639562368No Hit
ATGCTCTGTTGCCTGTCTCTTATACAC730.12325251570203283No Hit
ATGCTCTGTTGCGGGGGCTGTCTCTTA680.1148105625717566No Hit
ATGCTCTGTTGCGCCTGTCTCTTATAC680.1148105625717566No Hit
ATGCTCTGTTGCCCCTGTCTCTTATAC630.10636860944148038No Hit
ATGCTCTGTTGCGGGGGGGGTTTTTTT620.10468021881542514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTG601.0095391E-921.0000025
TTGCATG601.0095391E-921.0000029
TTGCAGA850.021.0000029
TTGCACT303.8731899E-421.0000029
AGCTTTT558.556526E-921.00000218
TTGCAAG751.8189894E-1221.0000029
TTGCAAC303.8731899E-421.0000029
TGCGCTG558.556526E-921.00000210
ACGTTTT601.0095391E-921.00000218
TGCGCCT601.0095391E-921.00000210
ATGATTT303.8731899E-421.00000217
AGGATTT303.8731899E-421.00000217
GGCGTTT558.556526E-921.00000217
TGCGTAG601.0095391E-921.00000210
TGCCGTA303.8731899E-421.00000210
TGCAAGG303.8731899E-421.00000210
TTTTCTG651.200533E-1021.00000221
TGCGAGG751.8189894E-1221.00000210
TGCGACT303.8731899E-421.00000210
TGCGAAG558.556526E-921.00000210
Produced by FastQC (version 0.11.2)