FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_13.3510000005b43a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_13.3510000005b43a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67649
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTATGGTCTGTCTCTTATACACA2560.378423923487413No Hit
GACTCTATGGTGGCTGTCTCTTATACA2450.3621635205250632No Hit
GACTCTATGGTGCCTGTCTCTTATACA1530.22616742302177414No Hit
GACTCTATGGTGTCTGTCTCTTATACA1180.1744297772324794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTT250.003347425321.00000216
TGGTTCG250.003347425321.00000211
GGTGAGA1100.021.0000029
GGTGACT751.8189894E-1221.0000029
GGCATTT250.003347425321.00000217
AGCTTTT507.300696E-821.00000218
GTGCAGG250.003347425321.00000210
TCGATTT250.003347425321.00000217
GCAAGTT250.003347425321.00000216
GAAATTT250.003347425321.00000217
TGGTATT250.003347425321.00000216
AATTCTG250.003347425321.00000219
GGACTTT250.003347425321.00000217
GTACTGT250.003347425321.00000218
AGGGTTT250.003347425321.00000217
CAATTTT558.611096E-921.00000218
GAGCTTT250.003347425321.00000217
GTTTCTG751.8189894E-1221.00000220
TGTTTCG250.003347425321.00000211
TCAATTT250.003347425321.00000217
Produced by FastQC (version 0.11.2)