FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_11.3510000005b3f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_11.3510000005b3f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172146
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCCTCAGCTGTCTCTTATACACA3110.18066060204709958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTCTG1000.021.02589620
ATTTTCT303.882447E-421.02589620
AGCTTTT1100.021.0075618
TGCGTTT558.811185E-921.0075617
CCTGTTT558.811185E-921.0075617
GAAGTTT558.811185E-921.0075617
CTGGTTT558.811185E-921.0075617
CGTGTTT558.811185E-921.0075617
AGCGTTT558.811185E-921.0075617
TACTTTT1100.021.0075618
CAAATTT558.811185E-921.0075617
TTCATTT601.0459189E-921.00755917
CCAATTT456.29876E-721.00755917
GGGTTTT4000.021.00755918
AATCTTT303.9054037E-421.00755917
GGCATTT601.0459189E-921.00755917
ATCTTTT1150.021.00755918
GAAATTT507.4398486E-821.00755917
CGCCTTT507.4398486E-821.00755917
GCGATTT303.9054037E-421.00755917
Produced by FastQC (version 0.11.2)