FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n01_kb_plate1_10.3510000005b3db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n01_kb_plate1_10.3510000005b3db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69228
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC5270.7612526723291154No Hit
ACGTCTCTGCCTGTCTCTTATACACAT2280.3293465071936211No Hit
ACGTCTCTGCATGGGGGCTGTCTCTTA840.12133818686080777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTGT751.8189894E-1221.04855220
TTTTCTG507.130075E-821.04855221
GTTTTCT303.822128E-421.04855220
TCTACAC303.822128E-421.04855221
TTCTACA303.822128E-421.04855220
CTTTCTG456.071896E-721.0485520
GGGTTTT3400.021.01809118
AAATTTT850.021.01809118
TGCGTTT250.003331496421.01809117
AATTCTG303.8597675E-421.01809119
TTTCTTT507.240487E-821.01809117
AGGATTT250.003331496421.01809117
CCTGTTT250.003331496421.01809117
AATTTCT250.003331496421.01809119
TTATTTT850.021.01809118
TGATTTT507.240487E-821.01809118
CTCTTTT250.003331496421.01809118
GATGTTT601.0077201E-921.01809117
TGTTTTT5800.021.01809119
TACATTT250.003331496421.01809117
Produced by FastQC (version 0.11.2)