FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n02_yp2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n02_yp2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781796
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCG1105.518754E-639.2729539
TGAGCCG1303.902951E-738.7694555
TAGGACA2800.036.000214
CTGGGCA3300.034.909299
CTGTGCG1459.1784386E-734.7588239
TAGACCA2501.8189894E-1234.56025
TCGCGCG850.002836147833.882559
TAGGCTA1751.0718759E-732.9144745
GGCCTAG1102.535606E-432.7295571
CTCGCTC1352.2452841E-532.0001878
CGGCCCG950.004889253530.3159649
CCGCGCC950.004889253530.3159649
CCCGAGA950.004889253530.3159644
CTTAGGA3101.8189894E-1230.1937222
GTATTAT5250.030.1735341
ACCGCGC1204.224963E-430.0001748
TAGGTAG2253.011155E-828.8001675
TTAGTCC2003.455716E-728.8001653
CTAGGAT1504.6098052E-528.8001653
GTCTACA6800.028.5902291
Produced by FastQC (version 0.11.2)