FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n02_yp1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n02_yp1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004326
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG3200.033.750645
GTCCTAT2258.4946805E-1032.011771
CTACACT5200.031.846764
TAGACAG3000.031.2005925
TGGACCG2809.094947E-1230.8577295
TATAGTG3800.030.3163685
ACCACGG1703.1768795E-629.64918
GTCGTGC2452.1209416E-929.389788
GTATAGG4450.029.1343041
CCTAAGG3550.028.3963223
ACTGCTC5150.027.9630898
TCCTAAG4400.027.8214822
GTCCAAG4150.027.7692451
CTGGACC4700.027.5749914
TAAGGTG5500.027.4914325
CCTAGGA1358.4102876E-426.669832
GGTGCGC1358.4127346E-426.6685038
GTAGTAC2705.984475E-926.6685033
CTAGGGG1907.546574E-626.5281433
TATACAG6300.026.2862135
Produced by FastQC (version 0.11.2)