FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n02_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n02_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462023
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAAGC2705.456968E-1231.9899963
ATAGGAC1652.5210138E-630.5359063
TCGGTCG1952.7618444E-729.5420139
TAATACT4000.028.8003484
CTATCCC2253.0053343E-828.8003484
GGATCGG2253.0053343E-828.8003486
TAGGACA2502.6266207E-928.8003464
GTCTACA4800.028.4910891
TCTATCC3103.092282E-1127.8622553
TAGACAG3650.027.6167725
CCTAAGG1358.4245787E-426.6583293
CTATAAA3006.366463E-1026.3917471
GTATAGA3701.0913936E-1125.2893891
ATACTGG2951.5321348E-824.4070746
AATACAG3251.6025297E-924.3695245
ATAATAC4750.024.245053
GATCGGT2701.7583989E-724.000297
CTATACA2752.0993139E-723.563924
GACCGTC2452.0265325E-623.5104877
TGTACCG1851.916237E-423.3516335
Produced by FastQC (version 0.11.2)