FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n02_mat0a1_18-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n02_mat0a1_18-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26514028
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG586310.22113199850283027No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA369530.13937150552907315No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT282670.10661148883149706No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT266330.10044871341314116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGG623650.028.193176
TATACAG756200.027.1458235
TACAGGC401350.027.0528077
GAGCGCA122700.025.7021569
TAGAGCG125500.025.6447267
ACAGGCA431800.024.7785668
TGCAGTC154500.024.279717
CAGGCAA467600.022.4349489
TACAGGT305000.021.5056617
GTATATA949000.020.9500221
ACAGGTT358350.018.5453198
ATTATAC552400.018.4566843
TATATAC1132550.018.0807463
CTCTGTG224650.017.9479927
TCTATAC265200.017.2674313
GTATTAG251750.017.018911
ATATACA1113950.016.9348184
TATACCG192600.016.8228175
CAGTCTC251300.016.3027089
TATACGG173750.016.2029615
Produced by FastQC (version 0.11.2)