FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n02_mah58_18-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n02_mah58_18-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28626859
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG1277220.44616141784888097No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA1031850.3604482070491911No Hit
TTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCCCAGGT622680.21751600481212416No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT535730.18714243151859586No Hit
GTTATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAG511370.1786329404843193No Hit
CTATAATGCAGTCTCTTGATAACTTTTTGCACTGTAGGTCCGTTAAGGTT445620.15566499978219756No Hit
ATATATTATTATACAGGTTCAAATATACTATCTGTTTCAGGGAAAACTCC442940.1547288160395103No Hit
ATATAGGGATATAGCACAGAGATATATAGCAAAGAGATACTTTTGAGCAA430770.1504775637452925No Hit
GCATTATAGAGCGCACAAAGGAGAAAAAAAGTAATCTAAGATGCTTTGTT427930.14948548843587764No Hit
ATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCT423880.14807073315308536No Hit
GATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTC382960.1337764649625025No Hit
ATATAGAACAGCTAAAGGGTAGTGCTGAAGGAAGCATACGATACCCCGCA371310.12970686026014938No Hit
GTATAATAATATATAGTCTAGCGCTTTACGGAAGACAATGTATGTATTTC334610.11688673214200693No Hit
GATATAGGTGCGACGTGAACAGTGAGCTGTATGTGCGCAGCTCGCGTTGC322670.11271582397496002No Hit
TATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGC318990.11143031794022529No Hit
GTATATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTG308420.10773798131328344No Hit
CTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACT302780.10576780358613566No Hit
ATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTATTCGCA290240.10138730204386029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAGTC215750.039.5467537
TAGAGCG219750.033.8793377
GAGCGCA222100.032.9700979
ATACAGG1084350.031.0420846
TACAGGC716200.030.2604567
TATACAG1181800.030.1949565
ACAGGCA735150.029.1770678
TACAGGT439250.026.866467
ATAGAGC305650.025.9830346
CAGGCAA820200.025.9672669
CTCTGTG337600.025.017217
ACAGGTT477200.024.775358
TAATGCA448900.024.5731054
GTATATA1405600.024.508331
TATACCG251950.023.7483655
ATGCAGT383450.023.5465626
TCTATAC296850.023.3338623
CAGTCTC385450.023.2007169
ATTATAC646750.023.1789043
GTATAAT436300.022.7265221
Produced by FastQC (version 0.11.2)