FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n02_mah4_18-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n02_mah4_18-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20417359
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA343780.16837633114057504No Hit
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG309400.15153771846789782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGG376900.026.4962966
TATACAG462150.026.2827765
TGCAGTC110100.024.5886297
TACAGGC231400.024.1452757
ACAGGCA255700.021.8790448
GTATATA696350.021.4050871
GAGCGCA78100.021.3880279
TACAGGT210950.020.4447157
TAGAGCG83600.020.0670457
TATACCG147700.018.7680595
TCTATAC209850.018.7680243
CAGGCAA295000.018.5492089
CTCTGTG171800.018.482017
ATTATAC393200.018.3116153
GTATTAG195650.017.6658941
ACAGGTT250750.017.1423938
GTATAGA227500.017.0917511
TATACTG236950.016.621485
CAGTCTC182650.016.5563059
TATATAC812400.016.4581953
Produced by FastQC (version 0.11.2)