FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n02_mah148_18-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n02_mah148_18-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32074263
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG586080.18272594447454646No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA394450.12298022249178414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAGTC190850.027.2762287
ATACAGG665850.026.7088586
TATACAG819050.026.091345
TACAGGC431500.025.496517
GAGCGCA135500.024.6029979
TAGAGCG140250.024.0773227
ACAGGCA468900.023.294378
CAGGCAA516350.020.5959879
GTATATA1175350.020.119451
CTCTGTG275700.019.4039527
TATACCG242100.019.2718375
TCTATAC343900.019.2407593
ATTATAC655650.018.9323273
TACAGGT340400.018.9097657
CAGTCTC297750.018.741159
TATATAC1359450.017.5202833
GTATAAT540250.017.487161
GTATTAT784850.017.3310741
ATGCAGT344150.017.3227716
GCAGTCT307450.017.2599148
Produced by FastQC (version 0.11.2)