FastQCFastQC Report
Thu 12 Apr 2018
HT7H3AFXX_n02_mah137_18-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT7H3AFXX_n02_mah137_18-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30997457
Sequences flagged as poor quality0
Sequence length150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTG579430.18692823737121403No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCGTGTTTATGCTTAAA428720.13830811992093414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGG629100.026.7698126
TACAGGC410150.026.2091277
TATACAG785550.025.7557135
TGCAGTC178550.024.4773437
ACAGGCA447250.023.7779678
GAGCGCA128400.022.6550169
TAGAGCG133450.022.5524227
CAGGCAA496200.021.0406579
GTATATA1117300.020.249381
TACAGGT320100.018.286917
TATACCG228500.018.181545
TCTATAC327000.017.3727343
TATATAC1327900.016.8738123
ATTATAC624150.016.8538913
CAGTCTC281500.016.6513969
GCAGTCT280350.016.1545438
ATGCAGT321100.015.8082426
GTATTAG301700.015.7761691
TATACTG361250.015.7257085
GTATAAT522550.015.681581
Produced by FastQC (version 0.11.2)